OBJECTIVE

PROUD 4, an upgraded formula of PROUD 3®, was tested against various pathogens in a lab assay to determine effectiveness of control.

MATERIALS & METHODS

In an independent, on-site laboratory in Florida, petri-disks inoculated with pathogens were prepared: alternaria (Alternaria solani), anthracnose (Colletotrichum sp.), botrytis (Botrytis cinera), fusarium (Fusarium sp.), macrophomina, (Macrophomina phaeseolina), neopestalotiopsis (Neopestalotiopsis sp.), phytopthora (Phytophthora cactorum), pythium (Pythium sp.), rhizoctonia (Rhizoconia solani), citrus canker (Xanthomonas citri), citrus greening (CLAS, Candidatus Liberibacter asiaticus), and bacterial canker (Pseudomonoas syringae). Petri-disks were treated once with Proud 4 at rates 1% v/v, 2% v/v, and 4% v/v. An untreated check was also included for reference. Plots were replicated eight times in a randomized complete block design.

For mycelial disk diameter assays, product was applied to the media, with inoculation following to measure growth (mm) (See bar graphs below). For CFU assays, pathogens were inoculated via streaking. Product was directly added to the media before CFU (#/plot) counts. All data was recorded and analyzed in ARM Rev. 2026.1. Statistics were analyzed using ANOVA mean comparison with LSD test, a=0.05.

RESULTS

PROUD 4 exhibited complete control for Alternaria, Anthracnose, Fusarium, Macrophomina, Neopestalotiopsis, Pythium, and Rhizoctonia. It had a very positive effect on Botrytis, Citrus Greening Canker, Citrus Canker, and Bacterial Canker.

PROUD 4 vs. ALTERNARIA

PROUD 4 vs. ALTERNARIA

PROUD 4 vs. ANTHRACNOSE

PROUD 4 vs. ANTHRACNOSE

PROUD 4 vs. BOTRYTIS

PROUD 4 vs. BOTRYTIS

PROUD 4 vs. FUSARIUM

PROUD 4 vs. FUSARIUM

PROUD 4 vs. MACROPHOMINA

PROUD 4 vs. MACROPHOMINA

PROUD 4 vs. NEOPESTALOTIOPSIS

PROUD 4 vs. NEOPESTALOTIOPSIS

PROUD 4 vs. PHYTOPTHORA

PROUD 4 vs. PHYTOPTHORA

PROUD 4 vs. PYTHIUM

PROUD 4 vs. PYTHIUM

PROUD 4 vs. RHIZOCTONIA

PROUD 4 vs. RHIZOCTONIA

PROUD 4 vs. CITRUS CANKER

PROUD 4 vs. CITRUS CANKER

PROUD 4 vs. CITRUS GREENING

PROUD 4 vs. CITRUS GREENING

PROUD 4 vs. BACTERIAL CANKER

PROUD 4 vs. BACTERIAL CANKER