Bio Energizer® is frequently used to facilitate bioremediation of wastewater. A study was conducted by an independent laboratory to measure possible negative effects Bio Energizer® might have on a freshwater test species (rainbow trout).  

Using EPA-approved methodology to evaluate Bio Energizer®, the lab administered the product at 10 ppm to a test tank and a control tank over a 96-hour period. It was concluded that Bio Energizer® had no negative effect on the health or mortality of the test species. 


An independent laboratory conducted a Whole Effluent Toxicity (WET) test on Bio Energizer®, using rainbow trout (oncorhynchus mykiss) as the freshwater test species. EPAapproved* Method 2019.0, which includes acute toxicity testing LC-50 (percent effluent concentration that is lethal to 50% of the test organisms).  

A glass tank with a disposable plastic liner was used to hold the trout and 20 liters of ammonia-free, dechlorinated water, to which Bio Energizer® was added at a concentration of 10 ppm (most fish-farming activities do not use concentrations higher than 7 ppm). A control tank was also set up and monitored that did not include Bio Energizer® (0 ppm). The two tanks were monitored, and measurements were taken at the start and at 0.25 hours, 0.50 hours, 1.0 hour, 2.0 hours, 4.0 hours, 24.0 hours, 48.0 hours, 72.0 hours, and 96.0 hours (see Summary Table 1.) 


Over a 96-hour testing period, it was determined that Bio Energizer® administered to water at a concentration of 10 ppm had no measurable acute negative effects on the health or mortality of rainbow trout. 

* Biological testing has long been used by the U.S. Environmental Protection Agency (EPA) as a method of identifying potentially toxic pollutants to the nation’s waterways. The requirements for testing are regulated in the Clean Water Act, Section 304 (h). Standard methods titled Whole Effluent Testing (WET) are specified in 40 CFR 136.6, Table I A, of the Code of Federal Regulations (CFR). 

Summary Table 1. 96-Hour Rainbow Trout Bioassay Report

Product Information 

Bio Energizer® is a formulation of nutrients, organic acids, natural biological stimulants, and energy systems that balance the natural microbial ecosystem to increase bio-oxidation capacity in lagoon systems. Bio Energizer® is a broad-spectrum bio-activator containing over 30 essential microbial growth-promoting ingredients. By design, Bio Energizer® is a balanced formulation of vitamins, trace nutrients, enzymes, organic acids, and biostimulants that stimulate the existing microbial community to greater metabolic capacity and efficiency. Bio Energizer® is neither a bacterium nor an inoculum. For more information, go to www.huma.us.