Dr. Richard T. Lamar and Dr. Hiarhi Monda of our Humic Research Laboratory, with assistance from analytical chemist Ryan Fountain, have published a methodology video in the biochemistry section of the peer-reviewed online video journal, JoVE.
The video, Quantification of Humic and Fulvic Acids in Humate Ores, DOC, Humified Materials and Humic Substance-Containing Commercial Products, shows the step-by-step laboratory methodology (the New Standard Method) for gravimetric quantification of humic substances (e.g., humic and fulvic acids) on an ash-free basis, in dry and liquid materials from soft coals (i.e., oxidized and non-oxidized lignite and sub-bituminous coal), humate ores and shales, peats, composts and commercial fertilizers and soil amendments.
In the video introduction, Dr. Lamar states, “The New Standard Method for quantification of humic acids provides a more accurate and precise analysis compared to the existing regulatorily accepted methods, and it also provides a standard method for pure hydrophobic fulvic acid quantification. The advantage of this protocol is that it provides a gravimetric analysis of humic and hydrophobic fulvic acid concentrations on an ash-free basis, and the extraction process has been optimized to obtain the highest recoveries of both humic and fulvic acids from samples.
At the video’s conclusion, Dr. Monda states, “Following this procedure, the dry humic and fulvic acids obtained can be used for characterization purposes, such as the carbon-13 and the proton NMR electron resonance, and the ultrahigh resolution mass spectrometry, among other useful techniques. This can be used for characterization of the humus chemistry, as well as being a useful tool to dig deep into the structure-activity relationship with plant fitness and the underlying plant defense mechanisms.
Direct link to video on the JoVE Website: https://www.jove.com/v/61233/quantification-humic-fulvic-acids-humate-ores-doc-humified-materials (A free subscription will be required to view the entire video on the JoVE Website.)
From the JoVE Website: Filmed at the world’s top scientific institutions, JoVE videos bring to life the intricate details of cutting-edge experiments enabling efficient learning and replication of new research methods and technologies. JoVE is a peer-reviewed scientific video journal that is indexed in PubMed and Web of Science.
Related Posts
BHN Hosted Annual Staff Meeting and Awards Night in Arizona
Bio Huma Netics, Inc. (BHN) hosted its annual staff meeting on December 14-15, 2022, at its headquarters in Gilbert, Arizona. BHN staff from New Mexico, Mexico, and Brazil also flew in to attend the two-day event. The company planned the all-staff gathering to recognize employees’ contributions and to celebrate significant milestones achieved in 2022—including the
This Week in Ag #22
Twenty years ago, I found myself in the position of operating a farm all by myself. My father, who I was farming with, passed away suddenly that January. So in addition to my full-time ag marketing job, I was flying solo across my family farm in western Illinois.
It All Has to Start With The Soil
The Soil Health Institute has released a 60-minute documentary, Living Soil, that captures the history — and significance — of the soil health movement. Our soils support 95 percent of all food production, and by 2060, our soils will be asked to give us as much food as we have consumed in the last 500
