Dr. Richard T. Lamar and Dr. Hiarhi Monda of our Humic Research Laboratory, with assistance from analytical chemist Ryan Fountain, have published a methodology video in the biochemistry section of the peer-reviewed online video journal, JoVE.
The video, Quantification of Humic and Fulvic Acids in Humate Ores, DOC, Humified Materials and Humic Substance-Containing Commercial Products, shows the step-by-step laboratory methodology (the New Standard Method) for gravimetric quantification of humic substances (e.g., humic and fulvic acids) on an ash-free basis, in dry and liquid materials from soft coals (i.e., oxidized and non-oxidized lignite and sub-bituminous coal), humate ores and shales, peats, composts and commercial fertilizers and soil amendments.
In the video introduction, Dr. Lamar states, “The New Standard Method for quantification of humic acids provides a more accurate and precise analysis compared to the existing regulatorily accepted methods, and it also provides a standard method for pure hydrophobic fulvic acid quantification. The advantage of this protocol is that it provides a gravimetric analysis of humic and hydrophobic fulvic acid concentrations on an ash-free basis, and the extraction process has been optimized to obtain the highest recoveries of both humic and fulvic acids from samples.
At the video’s conclusion, Dr. Monda states, “Following this procedure, the dry humic and fulvic acids obtained can be used for characterization purposes, such as the carbon-13 and the proton NMR electron resonance, and the ultrahigh resolution mass spectrometry, among other useful techniques. This can be used for characterization of the humus chemistry, as well as being a useful tool to dig deep into the structure-activity relationship with plant fitness and the underlying plant defense mechanisms.
Direct link to video on the JoVE Website: https://www.jove.com/v/61233/quantification-humic-fulvic-acids-humate-ores-doc-humified-materials (A free subscription will be required to view the entire video on the JoVE Website.)
From the JoVE Website: Filmed at the world’s top scientific institutions, JoVE videos bring to life the intricate details of cutting-edge experiments enabling efficient learning and replication of new research methods and technologies. JoVE is a peer-reviewed scientific video journal that is indexed in PubMed and Web of Science.
Related Posts
This Week in Ag #41
This is American agriculture’s big week – Thanksgiving! Our celebration of food takes center stage on family dining tables from sea to shining sea. Not only do we honor the 1% who currently feed us, we also reflect upon the many contributions of the original American agriculturalists, our Native Americans. For starters, they saved the Pilgrims from starvation during their first years in the New World. The Wamponoag tribe utilized their famous “Three Sisters” cropping practice: corn, beans and squash.
BIO ENERGIZER® Reduces Sludge 45% In One Year, Saves Municipal Plant $6 Million In Dredging Costs
by Heather Jennings, PS In this study, a one-year bioremediation plan featuring Bio Energizer® was implemented for a municipal wastewater treatment facility with 2 primary lagoons in which sludge depths had reached 5–7 feet. The lagoons were at risk of upset and wastewater processing capacity was reduced. Bio Energizer® was added via peristaltic pump to
This Week in Ag #54
Farmers love to kick tires, share stories, and due to their curious nature, are always looking to pick up tips for their trade. It’s no wonder the agricultural industry is rife with trade shows. Perhaps the most influential show is Commodity Classic, where the National Corn Growers Association, American Soybean Association, National Wheat Growers Association
