Dr. Richard T. Lamar and Dr. Hiarhi Monda of our Humic Research Laboratory, with assistance from analytical chemist Ryan Fountain, have published a methodology video in the biochemistry section of the peer-reviewed online video journal, JoVE.
The video, Quantification of Humic and Fulvic Acids in Humate Ores, DOC, Humified Materials and Humic Substance-Containing Commercial Products, shows the step-by-step laboratory methodology (the New Standard Method) for gravimetric quantification of humic substances (e.g., humic and fulvic acids) on an ash-free basis, in dry and liquid materials from soft coals (i.e., oxidized and non-oxidized lignite and sub-bituminous coal), humate ores and shales, peats, composts and commercial fertilizers and soil amendments.
In the video introduction, Dr. Lamar states, “The New Standard Method for quantification of humic acids provides a more accurate and precise analysis compared to the existing regulatorily accepted methods, and it also provides a standard method for pure hydrophobic fulvic acid quantification. The advantage of this protocol is that it provides a gravimetric analysis of humic and hydrophobic fulvic acid concentrations on an ash-free basis, and the extraction process has been optimized to obtain the highest recoveries of both humic and fulvic acids from samples.
At the video’s conclusion, Dr. Monda states, “Following this procedure, the dry humic and fulvic acids obtained can be used for characterization purposes, such as the carbon-13 and the proton NMR electron resonance, and the ultrahigh resolution mass spectrometry, among other useful techniques. This can be used for characterization of the humus chemistry, as well as being a useful tool to dig deep into the structure-activity relationship with plant fitness and the underlying plant defense mechanisms.
Direct link to video on the JoVE Website: https://www.jove.com/v/61233/quantification-humic-fulvic-acids-humate-ores-doc-humified-materials (A free subscription will be required to view the entire video on the JoVE Website.)
From the JoVE Website: Filmed at the world’s top scientific institutions, JoVE videos bring to life the intricate details of cutting-edge experiments enabling efficient learning and replication of new research methods and technologies. JoVE is a peer-reviewed scientific video journal that is indexed in PubMed and Web of Science.
Related Posts
Our Most Popular Case Studies
Bioremediation can improve the activity and reproduction of wastewater microbiology. The following case studies used bioremediation to improve wastewater treatment conditions and operating costs.
This Week in Ag #37
One of the greatest inventions in history is the combine. The concept of threshing and separating grain in one operation revolutionized our food system, as well as redefined our labor force. Consider that in the mid-1800s, 90% of the US workforce was involved in some aspect of farming. Now it’s under 2%. To think my grandfather harvested corn by hand and threw the ears in a wagon! He used the pull-behind model in the 1940s to harvest small grains (that’s him, Fred Nichols, combining oats on our family farm). My mother still talks about dad wearing a Jesse James style mask while operating their first self-propelled combine without a cab.
BHN Earns 2019 Biostimulant Industry Impact Award
Bio Huma Netics, Inc. (BHN), located in Gilbert, Arizona, is the recipient of the 2019 Biostimulant Industry Impact Award. The award was presented at Biostimulant CommerceCon in Atlantic City, New Jersey, by AgriBusiness Global and its parent company, Meister Media Worldwide. This prestigious annual award is given to the company that a panel has judged...